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TET1 is a tumor suppressor of hematopoietic malignancy
(Nature Publishing Group, 2015-04)
The methylcytosine dioxygenase TET1 (‘ten-eleven translocation 1’) is an important regulator of 5-hydroxymethylcytosine (5hmC) in embryonic stem cells. The diminished expression of TET proteins and loss of 5hmC in many ...
Human T Cells Expressing a CD19 CAR-T Receptor Provide Insights into Mechanisms of Human CD19-Positive β Cell Destruction
(Elsevier BV, 2020)
© 2020 Autoimmune destruction of pancreatic β cells underlies type 1 diabetes (T1D). To understand T cell-mediated immune effects on human pancreatic β cells, we combine β cell-specific expression of a model antigen, CD19, ...
Phosphorylation of MeCP2 at Serine 80 regulates its chromatin association and neurological function
(National Academy of Sciences, 2009-02)
Mutations of MECP2 (Methyl-CpG Binding Protein 2) cause Rett syndrome. As a chromatin-associated multifunctional protein, how MeCP2 integrates external signals and regulates neuronal function remain unclear. Although ...
Pan-Src Family Kinase Inhibitors Replace Sox2 during the Direct Reprogramming of Somatic Cells
(Wiley Blackwell (John Wiley & Sons), 2011-05)
Small molecules do the job: Somatic cells are reprogrammed into iPS cells upon ectopic expression of Oct4, Sox2, Klf4 and c-Myc. Application of a cell-based, high-throughput chemical screen led to the identification of Src ...
Musashi-2 regulates normal hematopoiesis and promotes aggressive myeloid leukemia
(Nature Publishing Group, 2010-07)
RNA-binding proteins of the Musashi (Msi) family are expressed in stem cell compartments and in aggressive tumors, but they have not yet been widely explored in the blood. Here we demonstrate that Msi2 is the predominant ...
Genetic engineering of human ES and iPS cells using TALE nucleases
(Nature Publishing Group, 2011-07)
Targeted genetic engineering of human pluripotent cells is a prerequisite for exploiting their full potential. Such genetic manipulations can be achieved using site-specific nucleases. Here we engineered transcription ...
Pluripotency and Cellular Reprogramming: Facts, Hypotheses, Unresolved Issues
(Elsevier B.V., 2010-11)
Direct reprogramming of somatic cells to induced pluripotent stem cells by ectopic expression of defined transcription factors has raised fundamental questions regarding the epigenetic stability of the differentiated cell ...
Generation of Isogenic Pluripotent Stem Cells Differing Exclusively at Two Early Onset Parkinson Point Mutations
(Elsevier, 2011-07)
Patient-specific induced pluripotent stem cells (iPSCs) derived from somatic cells provide a unique tool for the study of human disease, as well as a promising source for cell replacement therapies. One crucial limitation ...
One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering
(Cell Press/Elsevier, 2013-05)
Mice carrying mutations in multiple genes are traditionally generated by sequential recombination in embryonic stem cells and/or time-consuming intercrossing of mice with a single mutation. The CRISPR/Cas system has been ...
Partial FMRP expression is sufficient to normalize neuronal hyperactivity in Fragile X neurons
(Wiley, 2020)
© 2019 The Authors. European Journal of Neuroscience published by Federation of European Neuroscience Societies and John Wiley & Sons Ltd. Fragile X syndrome (FXS) is the most common genetic form of intellectual disability ...